Table 1.
Bacteria and phages used in this study.
| Bacteria/phage | Genotype or relevant characteristic a | Reference or source |
|---|---|---|
| Escherichia coli DH5α | supE44 lacU169(Ø80lacZΔ M15) hsdR17 ( ) recA1 endA1 gyrA96 thi‐1 relA1 | Woodcock et al. (1989) |
| E. coli CC118λpir | araD, Δ(ara, leu), ΔlacZ74, phoA20, galK, thi‐1, rspE, rpoB, argE, recA1, λpir | Herrero et al. (1990) |
| E. coli HH26 | Mobilizing strain for conjugal transfer | Kaniga et al. (1991) |
| E. coli β2163 | F‐ RP4‐2‐Tc::Mu ΔdapA::(erm‐pir); KmR EmR | Demarre et al. (2005) |
| Serratia plymuthica A153 | Wild type, rhizosphere isolate | Hökeberg et al. (1997) |
| LacZ | A153 ΔlacZ (1470 bp Δ) | Matilla et al. (2015) |
| VN1 | A153 transposon mutant admH::Tn‐KRCPN1lacZ; KmR | This study |
| VN2 | A153 transposon mutant admK::Tn‐KRCPN1lacZ; KmR | This study |
| VN3 | A153 transposon mutant admO::Tn‐KRCPN1lacZ; KmR | This study |
| VN7 | A153 transposon mutant admM::Tn‐KRCPN1; KmR | This study |
| VN9 | A153 transposon mutant admJ::Tn‐KRCPN1; KmR | This study |
| VN11 | A153 transposon mutant admC::Tn‐KRCPN1lacZ; KmR | This study |
| VN12 | A153 transposon mutant admP::Tn‐KRCPN1; KmR | This study |
| VN17 | A153 transposon mutant admO::Tn‐KRCPN1; KmR | This study |
| VN21 | A153 transposon mutant admK::Tn‐KRCPN1; KmR | This study |
| VN22 | A153 transposon mutant admP::Tn‐KRCPN1lacZ; KmR | This study |
| VN23 | A153 transposon mutant admF::Tn‐KRCPN1; KmR | This study |
| VN24 | A153 transposon mutant admE::Tn‐KRCPN1lacZ; KmR | This study |
| VN26 | A153 transposon mutant admS::Tn‐KRCPN1lacZ; KmR | This study |
| A153JH6 | A153 ΔlacZ, zmn13::Tn‐KRCPN1; Zeamine‐; KmR | Hellberg et al. (2015) |
| ANDV | A153 ΔadmV (336 bp Δ) | This study |
| ANDW | A153 ΔadmW (150 bp Δ) | This study |
| ANDX | A153 ΔadmX (789 bp Δ) | This study |
| XJH6 | A153 ΔadmX, zmn13::Tn‐KRCPN1; andrimid‐, zeamine‐; KmR | This study |
| ARpoS | A153 rpoS::Km; KmR | Matilla et al. (2015) |
| AHfq | A153 Δhfq::Km (252 bp Δ); KmR | Matilla et al. (2015) |
| A153H | A153 Δhfq (252 bp Δ) | This study |
| A153HL | A153 ΔlacZ, Δhfq | This study |
| LVN2 | A153 ΔlacZ, admK::Tn‐KRCPN1lacZ; generated by transduction using ϕMAM1; KmR | This study |
| HLVN2 | A153 ΔlacZ, Δhfq, admK::Tn‐KRCPN1lacZ; generated by transduction using ϕMAM1; KmR | This study |
| XLVN2 | A153 ΔlacZ, ΔadmX, admK::Tn‐KRCPN1lacZ; generated by transduction using ϕMAM1; KmR | This study |
| ASptI | A153 in‐frame sptI (483 bp Δ) | Matilla and Salmond (unpubl. data) |
| ASptR | A153 sptR::Km; KmR | Matilla and Salmond (unpubl. data) |
| ASplR | A153 splR::Km; KmR | Matilla and Salmond (unpubl. data) |
| ASpsR | A153 spsR::Km; KmR | Matilla and Salmond (unpubl. data) |
| A153C | A153 in‐frame csrB (267 bp Δ) | Matilla and Salmond (unpubl. data) |
| Serratia marcescens MSU97 | Wild type, plant epiphyte, pigmented | Strobel et al. (1999) |
| Bacillus subtilis JH642 | pheA1 trpC2 | J.A. Hoch |
| Dickeya solani MK10 | Wild type, plant pathogen | Pritchard et al. (2013) |
| Xanthomonas campestris pv. campestris | Wild type, plant pathogen | R. Penyalver |
| Phages | ||
| ϕMAM1 | Generalized transducing phage for S. plymuthica A153 | Matilla and Salmond (2014) |
a. The following abbreviations are used: Km, kanamycin; Tc, tetracycline; Em, erythromycin.