Table 1.
Summary of sensitivity and maximum responses to methacholine in airway and parenchymal lung compartments. Summary of the threshold dose (sensitivity) required to elicit a detectable increase in airway resistance (Raw), tissue damping (G) and tissue elastance (H) for naïve mice, mice systemically sensitised with OVA/Alum and challenged with PBS and mice systemically sensitised and challenged with OVA. Also shown is the percentage change in Raw, G and H in response to the maximum does of methacholine used (30 mg.mL-1). Data are presented as the mean (SEM).
| Challenge | Assessed after last aerosol (hr) | Sensitivity - Threshold dose of MCh (mg.mL-1) | Response at 30 mg.mL-1 MCh | ||||||||||
| Raw | G | H | Raw§ | G§ | H§ | ||||||||
| p* | p* | p* | p* | p* | p* | ||||||||
| Naïve | - | 0.54(0.14) | - | 0.51(0.25) | - | 0.10(0.02) | - | 235.5(21.5) | - | 138.8(6.1) | - | 145.4(5.1) | - |
| 1 PBS aerosol | 24 and 48 pooled | 0.55(0.30) | ns | 0.25(0.10) | ns | 0.06(0.01) | ns | 242.9(19.1) | ns | 141.5(4.7) | ns | 143.4(2.7) | ns |
| 1 OVA aerosol | 24 | 0.09(0.03) | 0.012 | 0.15(0.04) | ns | 0.05(0.01) | ns | 514.0(82.7) | <0.05 | 275.5(30.6) | <0.001 | 250.2(24.5) | <0.001 |
| 48 | 0.46(0.19) | ns | 0.18(0.05) | ns | 0.17(0.07) | ns | 326.4(32.6) | ns | 213.0(14.6) | ns | 179.3(12.6) | ns | |
| 3 OVA aerosols | 24 | 0.12(0.03) | 0.012 | 0.35(0.20) | ns | 0.06(0.01) | ns | 271.2(43.1) | ns | 180.3(41.7) | ns | 178.9(39.9) | ns |
| 48 | 0.20(0.07) | 0.034 | 0.16(0.04) | ns | 0.08(0.01) | ns | 348.8(46.0) | ns | 243.6(42.7) | ns | 233.0(31.6) | 0.045 | |
| 6 OVA aerosols | 24 | 0.17(0.05) | 0.019 | 0.12(0.05) | ns | 0.06(0.01) | ns | 456.7(43.4) | <0.05 | 358.2(88.4) | <0.05 | 298.5(52.2) | 0.02 |
| 48 | 0.16(0.05) | 0.034 | 0.15(0.06) | ns | 0.06(0.01) | ns | 396.0(23.3) | <0.05 | 304.8(28.5) | <0.05 | 295.6(30.6) | 0.018 | |
§ expressed as a % of saline response
* vs naïve values