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. 2016 Jul 1;7(30):46899–46912. doi: 10.18632/oncotarget.10380

Figure 5. Phosphorylated ERK 1/2 is localized to cytosol, nucleus, and other subcellular compartments upon HNG treatment.

Figure 5

Representative western blots showing ERK1/2 subcellular localization (n = 3). (A) HEK293 cells were treated with 1μM HNG. Both cytosolic (15μg) and nuclear (15μg and 50μg) lysates were immunoblotted using phospho- and total-ERK antibody. (B) SH-SY5Y cells were treated with 100μM HNG. Mitochondria fractions were obtained 30min after HNG treatment. Cytosolic, nuclear, and mitochondrial (15μg) lysates were immunoblotted using phospho- and total-ERK antibody. Anti-GAPDH, Anti-Lamin B, anti-Tom20 antibody were used as the cytosolic, nuclear, mitochondrial fraction markers, respectively. The ratio is the fold change in phospho-MAPK normalized to total-MAPK compared to the cytosolic, time 0 phospho to total ratio.