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. 2016 Jul 9;7(30):46943–46958. doi: 10.18632/oncotarget.10504

Figure 4. Thiacremonone ameliorates MPTP-induced dopaminergic neurodegeneration.

Figure 4

Level of dopamine A. and DOPAC (which is metabolite of dopamine) B. in the 4 groups of mice brain were determined by HPLC. Method was as described detail in materials and methods section. The effect of thiacremonone on TH-positive neurons was measured by western blot and immunohistochemical analysis. Expression of TH was examined by specific antibodies in the brain striatum and substantia nigra C. The sections of mice striatum and substantia nigra D. incubated with anti-TH+ primary antibody and the biotinylated secondary antibody (n = 3). The representive stained tissues were viewed with a microscope (X50 or 200). Represents the number of TH-positive neuronal cells in substantia nigra and TH-positive density in striatum E. Total cell number counted in each whole section. Each value is presented as mean ± SD from 10 mice. *, p < 0.05 Significant difference from saline-injected mice and #, p < 0.05 Significant difference between the MPTP-injection groups.