Figure 4. ChIP followed by qPCR-analysis of SOX2-enrichment at the SOX2 binding sites within indicated genes.

3% input chromatin was used for normalization and an IgG antibody was used as negative ChIP control. In qPCR, oligonucleotides were used to amplify a PCR-fragment around the SOX2 binding site (on target) and a PCR-fragment within the same gene, but without a SOX2 binding site (off target).