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. 2016 May 30;7(30):47242–47251. doi: 10.18632/oncotarget.9725

Figure 5. Vaccination.

Figure 5

A. Knockdown of CRT or HMGB1 by using short interfering RNA (siRNA). CT26 cells were transiently transfected with siRNAs against CRT or HMGB1. CRT or HMGB1 protein expression was analyzed by western blotting at 48 hours after transfection. B, C. CT26 cells treated in vitro with G-chlorin-PDT were inoculated subcutaneously into BALB/c mice. After 7 days, mice were re-challenged with live CT26 cells. The percentages of tumor-free mice were pooled. Each group comprised ten mice. Significance was determined by the log-rank statistic. * P < 0.05, ** P < 0.01 relative to control. (B; CRT, C; HMGB1)