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. 2016 Jun 18;7(30):47444–47464. doi: 10.18632/oncotarget.10165

Figure 9. PI3K/AKT signaling pathway mediates Kidins220-induced tumorigenesis and angiogenesis in vivo.

Figure 9

(A–B) AKT knockdown reduced tumorigenesis in vivo. PC3 cells were transduced with the pCDH-shAKT (shAKT) or the pCDH-vector control (vector) for 72 hour and further re-suspended in serum-free medium. The sizes of tumors from nude mice were determined by two-dimensional caliper measurements. Scatter plots represent the weights of independent tumors from different groups. Data represent mean ± SD, each group with five tumors (n = 5). Two independent experiments were performed and similar results were obtained. (C) The hemoglobin level of the Matrigel plugs was determined with O.D. value at 540 nm. Data represent mean ± SD and each group with five tumors (n = 5). Three independent experiments were performed and similar results were obtained. (D) AKT knockdown significantly decreased VEGF expression detected by immunostaining in tumors from xonografted PC3 cells (P = 6.29 × 10−3) with representative immunostaining images (original magnification, ×100) shown on the left. ** and *** indicate P < 0.01 and P < 0.001 by Student's t-test, respectively.