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. 2016 Jun 21;7(30):47494–47510. doi: 10.18632/oncotarget.10202

Figure 1. Endogenous pyruvate from glycolysis is not sufficient to support 143B206 proliferation.

Figure 1

(A) Stoichiometric summary of glycolysis and NAD+ homeostasis in 143B206 cells. (B) 143B206 cells were seeded in 96-well microplates and cultured overnight, then the media with indicated concentrations of pyruvate were used to culture cells for additional 48 h. Cell numbers were measured using CyQUANT assay. The relative cell numbers were normalized with the initial number of plated cells. (C and D) Proliferation rates of 143B206 and the parental 143B cells in the presence or absence of 1 mM pyruvate for indicated time were measured. (E) Morphology of 143B and 143B206 cells cultured in the presence or absence of 1 mM pyruvate was observed with Zeiss Axioplan microscope at 400× magnification. Bars represent 100 μm. Error bars indicate SD of ≥ 5 replicates. **p < 0.01.