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. 2016 Jun 21;7(30):47494–47510. doi: 10.18632/oncotarget.10202

Figure 4. Effects of disruption of pyruvate-based biosynthesis on 143B206 cell proliferation.

Figure 4

(A) PC, (B) PDHA1, and (C) CS were knocked down in 143B206 cells with siRNA. qRT-PCR (Left top) and immunoblotting (Left bottom) were employed to determine the knockdown efficiency. Proliferation curves (Right) of the control and knockdown 143B206 cells in the presence of exogenous pyruvate were measured. For qRT-PCR, error bars indicate 95% confidence interval of triplicates. For proliferation curves, error bars indicate SD of ≥ 5 replicates. **p < 0.01.