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. 2016 Jun 21;7(30):47511–47525. doi: 10.18632/oncotarget.10203

Figure 4. Overexpression of K14/ARF6 in aggressive CD49f+/CD44+/CD24− single-cell derived clones.

Figure 4

(A) Sections of S2B11 aggressive clone spheres have higher expression of Ki-67, K14 and ARF6 compared to spheres of non-aggressive S2D10 clone. (B) Spheres of more aggressive (S2B11 and S2G7) and less aggressive (S2D10 and S2F10) clones were embedded into 3D matrigel and their invasiveness was monitored. Spheres of more aggressive clones formed protruding structures showing their invading capacity while the less aggressive clones failed to form such structures. Bar scale represents 100 μm. (C) Spheres were stained with antibodies against K14 (red) and ARF6 (green). S2B11 and S2G7 have elevated K14 and ARF6 expression levels compared to S2D10 and S2F10. (D) Zoomed in image of S2B11 shows that the leading cells are either K14 or ARF6 positive if they are not positive for both. S2D10 failed to form leading cells.