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. 2016 Jun 7;7(30):47586–47592. doi: 10.18632/oncotarget.9896

Figure 1. Cancer stem cells are more invasive through 3D collagen gel than non-stem cancer cells.

Figure 1

A. Individual migration tracks of nuclear positions as cells migrate through collagen towards the serum or EGF gradient (gradient source is to the right). B. Representative images of non-cancer stem cells (left) and cancer stem cells (right) as they migrate through collagen under EGF stimulation conditions. (Scale bars = 25 μm). C. Cell migration through 3D collagen gels was determined by tracking fluorescently labeled nuclei and the velocity was determined. CSCs are indicated with a “+” and non-SCCs are indicated with a “-“. D. The length of the longest protrusion was measured to determine the axial length of a cell as it migrated through the collagen gel. E. The percent nuclear translocation through 3 micron sized pores of a transwell membrane was determined by comparing the number of nuclei that crossed the membrane to the total number of cells plated. (* = P < 0.05, ** = P < 0.01, *** = P < 0.001)