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. 2016 Jun 24;7(30):47760–47776. doi: 10.18632/oncotarget.10283

Figure 4. Requirement of PPARγ for the miR-27a antagonism effect on downstream gene expressions in vitro.

Figure 4

(A) MiR-27ai attenuated the silencing effect of PPARγ siRNA on TGF-β1 expression (immunofluorescence, scale bar 100 μm) and (B) quantification analysis. (C) The restoration of PPARγ decreased the expression of TGF-β1, Smad3, CTGF, Fibronectin, and Collagen I by qRT-PCR and (D) Western blot analyses. Results are presented as mean ± SD of three independent experiments. *P < 0.05; #P < 0.001. NT: non-targeting; siRNA: small interfering RNA; miR-27ai: miR-27a inhibitor; p-SMAD3, phospho-SMAD3; t-SMAD3, total-SMAD3. (n = 6).