Figure 2. PLK1 inhibition is sufficient to induce cell cycle arrest and apoptosis in NSCLC cell lines that have undergone EMT.

HCC4006-ER2 (A) and HCC827-ER3 (B) cell lines were treated with 60 nM volasertib and/or 2 μM erlotinib or with vehicle controls and the cell cycle was analyzed with a BrdU FITC flow cytometry kit with 7AAD after 48 h. Apoptosis was assessed with an APO-BrdU TUNEL assay. *p < 0.05 compared with vehicle control after 72 hours.