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. 2016 Jun 30;7(30):48027–48037. doi: 10.18632/oncotarget.10338

Figure 3. Dragon overexpression inhibits oxaliplatin-induced apoptosis in CMT93 cells.

Figure 3

(A and B) Glucose-deprived control (nc-pLV) and Dragon-overexpressing (oe-Dra) CMT93 cells were treated with increasing doses of oxaliplatin for 24 h before the TUNEL assay was performed. TUNEL-positive cells are shown in green, and the nuclei are shown in blue (A). The percentages of TUNEL-positive cells over total cell numbers are presented (B). (C and D) Control and Dragon-overexpressing CMT93 cells cultured in low-glucose medium were treated with increasing doses of oxaliplatin for 24 h before the cells were stained with AnnexinV-FITC/PI. Cell apoptosis was analyzed by flow cytometry (C). The percentages of apoptotic cells is shown (D). The data are presented as the mean ± SD of three independent experiments. *P < 0.05 and **P < 0.01. (E) Effect of Dragon overexpression on cleaved caspase-3 and cleaved PARP levels in CMT93 cells. Control (pLV) and Dragon overexpressing (oeD) CMT93 cells cultured in low glucose medium were treated with increasing doses of oxaliplatin for 24 h before the cells were harvested for Western blotting to detect cleaved caspase-3, full length caspase-3, cleaved PARP and full length PARP. (F) Effect of Dragon-overexpression on JNK, p38 and Erk phosphorylation in the presence of oxaliplatin under a glucose deprivation condition. Control (pLV) and Dragon-overexpressing (oeDra) CMT93 cells cultured in low glucose medium were treated with increasing doses of oxaliplatin for 24 h before the cells were collected for Western blotting to detect p-JNK/JNK, p-p38/p38 and p-Erk/Erk.