Skip to main content
. 2016 Jun 30;7(30):48027–48037. doi: 10.18632/oncotarget.10338

Figure 4. Dragon knockdown increases oxaliplatin-induced apoptosis in CMT93 cells.

Figure 4

(A and B) Control (nc-pU6) and Dragon knockdown (si-Dra) CMT93 cells cultured in low glucose medium were treated with increasing doses of oxaliplatin for 24 h before the TUNEL assay was performed. TUNEL-positive cells are shown in green, and the nuclei are shown in blue (A). The percentages of TUNEL-positive cells is presented (B). (C and D) Control and Dragon-knockdown CMT93 cells cultured in low glucose medium were treated with increasing doses of oxaliplatin for 24 h before the cells were stained with AnnexinV-FITC/PI. Cell apoptosis was analyzed by flow cytometry (C). The percentages of apoptotic cells is shown (D). The data are presented as the mean ± SD of three independent experiments. *P < 0.05. (E) Effect of Dragon-knockdown on cleaved caspase-3 and cleaved PARP levels in CMT93 cells. Control (pU6) and Dragon-knockdown (siD) CMT93 cells cultured in low glucose medium were treated with increasing doses of oxaliplatin for 24 h before the cells were harvested for Western blotting to detect cleaved caspase-3, full-length caspase-3, cleaved PARP and full-length PARP. Tubulin was used as a loading control. (F) Effect of Dragon knockdown on JNK, p38 and Erk phosphorylation in the presence of oxaliplatin. Control (pU6) and Dragon-knockdown (siD) CMT93 cells cultured in low glucose medium were treated with increasing doses of oxaliplatin for 24 h before the cells were collected for Western blotting to detect p-JNK/JNK, p-p38/p38 and p-Erk/Erk. Tubulin was used as a loading control.