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. 2016 Jun 17;7(30):48093–48106. doi: 10.18632/oncotarget.10137

Figure 1. Stiffer environments robustly increase PRL signals to pERK1/2 and pFAK Y925, but only slightly increase signals to pSTAT5.

Figure 1

A-C. T47D cells were plated on 12, 25, or 75 kPa polyacrylamide gels coated with 200 μg/ml collagen-I, serum starved for 24 h, and treated ± PRL (4nM) for 15 min. Cell lysates were immunoblotted with the indicated antibodies. Top panels: Representative immunoblots. Bottom panels: Quantification of immunoblots by densitometry. Means ± S.E.M. n = 5. Different letters represent significant differences between treatments, p<0.05. D-F. T47D cells were plated and serum starved as in A-C, and treated ± PRL (4nM) for 24h. Specific transcripts were quantitated by RT-PCR as described in the Methods. Means ± S.E.M. n = 3. Different letters represent significant differences between treatments, p<0.05. G. T47D cells plated as in A-C were harvested after serum starvation. Cell lysates were immunoblotted with the indicated antibodies.