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. 2016 Jun 21;7(30):48193–48205. doi: 10.18632/oncotarget.10194

Figure 3. Suppression of endogenous MT2-MMP inhibits E-cadherin shedding in A2780 cancer cells.

Figure 3

A. RT-PCR analysis of MMP3, 7, 9, MT1- and MT2-MMP in the A2780 cancer cell line. B. Shed E-cadherin N-terminal fragments in the conditioned medium were examined by Immunoprecipitation and Western blotting using anti-N-terminal E-cadherin antibodies (right panel). Cells were lysed and actin was detected as a loading control (right panel). Ponceau staining of the membrane with samples from the conditioned medium was used as a loading control (left panel). C, D. The effect of 3 pairs of siRNAs against MT2-MMP was analyzed by Real-time PCR (C). The best inhibition was achieved by Si-1 (D). E. Shed E-cadherin in the conditioned medium was examined following knockdown of MT2-MMP using MT2 Si-1. Left panel, Ponceau staining. Right panel, Western blotting with anti-N-terminal E-cadherin antibody. Cells were lysed and actin was detected as loading control. Mean ± SEM, n = 3. *P < 0.05.