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. 2016 Jun 22;7(30):48296–48308. doi: 10.18632/oncotarget.10219

Figure 7. MSC-induced resistance is mediated by the release of IL-6.

Figure 7

A. Chemotherapy sensitive and resistant osteosarcoma samples were collected, and the mean density of IL-6 was detected by immunohistochemistry. B. Saos-2 cells were exposed to 20 μg/ml doxorubicin and then treated with different concentrations of IL-6 for 48 h. The relative caspase 3/7 activity in the tumor cells was examined. C. Saos-2 cells were exposed to 20 μg/ml doxorubicin and then treated with 20 ng/ml of IL-6 for 4 days. The relative caspase 3/7 activity was examined daily. D. Saos-2 cells were cultured in the presence or absence of MSCs CM and then treated with IL-6 neutralizing antibody. The number of viable cells was then assessed using the CCK-8 assay. E. Saos-2 cells were exposed to 20 μg/ml doxorubicin and then treated with IL-6 and/or AG490. The caspase 3/7 activity was tested. F. Graphical depiction of the hypothetical pathway governing MSC-induced osteosarcoma resistance. Activation by IL-6 from MSCs may regulate drug resistance by modulating the expression of multiple drug resistant genes in osteosarcoma cells. n=3; *, p<0.01.