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. 2016 Jun 24;7(30):48401–48411. doi: 10.18632/oncotarget.10269

Figure 1. Expression, purification and characterization of recombinant proteins.

Figure 1

A~C. Induced expression of CTP-FoxM1, FoxM1, CTP protein. MK: prestained protein; 1: non-induced crude; 2: induced crude; 3: supernatant of lysate; 4: precipitation of lysate; D~F. Purification of CTP-FoxM1, FoxM1, CTP protein. MK: prestained protein; 1: IPTG induced culture; 2: supernatant of centrifugal IPTG induced culture; 3: column flow-through; 4: binding buffer; 5: imidazole wash buffer; 6: 500 mM imidazole elution buffer; G. Identification of CTP-FoxM1 fusion protein. 1: prestained protein; 2: CTP-FoxM1 fusion protein; 3: FoxM1 protein; 4: CTP protein