Skip to main content
. 2016 Jun 24;7(30):48401–48411. doi: 10.18632/oncotarget.10269

Figure 4. DCs activated by recombinant antigens generated antigen-specific T cell response.

Figure 4

A. Effects of different DC vaccination on T lymphocytes proliferation. T lymphocytes derived from C57BL/6 mice were co-cultured with PBS-DC, CTP-FoxM1-DC, FoxM1-DC, and CTP-DC for three days. Reactive T cells and stimulated cells were in ratio of 1:2, 1:4, 1:10, and 1:20. B. Levels of IFN-γ from CD8+T cell co-cultured with DCs pulsed with different antigens. Each group of DCs was incubated with 1ug/ml CTP-FoxM1, FoxM1, CTP, or PBS for 48h. CD8+T cells derived from C57BL/6 mice spleen were co-cultured with different group of DCs for 48h. The supernatant were harvested and IFN-γ in the supernatant was analyzed by ELISA. *P<0.05, **P<0.01, ***, P<0.001 when compared with DCs pulsed with PBS, FoxM1, or CTP. The data shown are the mean of triplicate experiments; the bars represent the mean ± SD. n = 5 mice/group. C. Levels of TNF-α from CD8+T cell co-cultured with DCs pulsed with different antigens. TNF-α in the supernatant was also analyzed by ELISA. *P<0.05, **P<0.01, ***, P<0.001 when compared with DCs pulsed with PBS, FoxM1, or CTP. The data shown are the mean of triplicate experiments; the bars represent the mean ± SD. n = 5 mice/group.