Figure 5. Level of CTLs induced by DCs pulsed with different antigens.
Each group of mice was administrated for three times at weekly interval with DCs pulsed with CTP-FoxM1, FoxM1, CTP, or PBS. One week after the last administration, splenocytes isolated from immunized mice from each group mentioned above were co-cultured with Hepa1-6 cell. Different effector/target cell ratios were mixed for 24 h. The lysis of target cells was determined by LDH release. The experiments were performed in triplicate, and the bars represent the mean ±SD; *P<0.05; ** P<0.01 when compared with DCs pulsed with PBS, FoxM1, or CTP; n = 5 mice/group.