Figure 6. M1 plus H89 significantly reduces tumor size.
A and B. Measurement of the antitumor efficacy of M1 and/or H89 in vivo. Nude mice (NU/NU) bearing subcutaneous HCT-116 (A) and Capan-1 (B) tumors were treated with vehicle, H89 (10 mg/kg/day), M1 virus (2 × 107 PFU/day), M1 virus and H89 (n ≥ 7). Tumor growth was assessed by tumor volume measurement over time (mean ± SD). At experimental endpoints, mice were anesthetized and sacrificed. i.v., intravenously injection (tail vein); **P < 0.01, compared with the combination group. C. Treatment schedule for HCT-116 and Capan-1 tumor model. D and E. Protein expressions in tumor mass with Immunohistochemistry staining. Immunohistochemistry was performed to analyze the expression of Ki-67 and Cleaved-Caspase-3. Scale bars=50μM.