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. 2016 Jul 6;7(30):48533–48546. doi: 10.18632/oncotarget.10406

Figure 5. ΔNp63 activates VEGF-A secretion by STAT3 and IL-8 axis.

Figure 5

A. Inhibition of endogenous ΔNp63 abrogates STAT3 phosphorylation in canine osteosarcoma cell lines. After control or si-ΔNp63 transfection, cells were cultured in media containing 1% FBS for 48 hr and cell extracts were analyzed by western blot with antibodies as shown. B. Stable expression of ΔNp63 in D17 cells significantly increases STAT3 phosphorylation. Cells were cultured in media containing 1% FBS for 48 hr and cell extracts were analyzed by western blot with antibodies as shown. C. Inhibition of STAT3 by specific siRNA was verified by western blot analysis. D. Inhibition of STAT3 in canine osteosarcoma correlates with decreased VEGF-A secretion. VEGF-A production in the media was determined as described in Materials and Methods. F. Stable expression of ΔNp63 in D17 cells significantly increases IL-8 secretion. IL-6 and IL-8 ELISA Kits were purchased from R&D Systems and performed according to the manufacturer's instructions. E. IL-8 mediates VEGF-A secretion in canine osteosarcoma cells. Cells were treated either si-ΔNp63 or the control siRNA for 48 hr. Subsequently, VEGF-A production in the media was determined as described in Materials and Methods.