Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan;149(1):1–36. doi: 10.1085/jgp.201611654

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Pendin et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 8. — Mitochondrial [Ca²⁺] hotspots. Pixel by pixel correlation showing the presence of Ca²⁺ microdomains on mitochondrial outer membrane (OMM) upon stimulation. HeLa cells cotransfected with nuclear (H2BD1cpv)- and OMM (N33D1cpv)-targeted Cameleons were treated with 100 µM histamine where indicated by the arrow in A. (A) The average [Ca²⁺] rises, expressed as ΔR/R₀, of nucleus (green trace) and OMM (blue trace) are very similar. (B) The number and intensity of single pixels, expressed as ΔR_max/ΔR₀ of single pixels, of the nucleus (green) or OMM (blue) reached during the first 4 s after histamine stimulation were plotted. The Gaussian fit of the nuclear (green) and OMM (red) pixel distribution highlights the right tail caused by hot-spot formation on OMM upon cellular stimulation. (C) Yellow to red color representation of ΔR_max/R₀ spatial distribution of pixels in 2D (left) and 3D (right), superimposed to YFP fluorescence image. Only pixels that during the 4 s after the histamine challenge have a ΔR_max/R₀ exceeding by 125% the ΔR/R₀ of the whole compartment were color coded. Bar, 5 µm (from Giacomello et al. [2010] with permission from Elsevier).