Table 3. The effect of length and GC content of different portions of transcripts on TEI.
| Feature | 5′ UTR (bp)a | CDS (bp) | 3′ UTR (bp) | |
|---|---|---|---|---|
| Size (bp) | ||||
| Mean Median Correlation | 215 | 1234 | 433 | |
| 147 | 1095 | 354 | ||
| Negative | Negative | Optimal size 200–400 bp | ||
| GC content (%) | ||||
| Mean Median Correlation | 58.3 | 56.1 | 39.3 | |
| 59.2 | 54.0 | 39.1 | ||
| Weak negative | Positive | Weak positive | ||
UTR, untranslated region; CDS, coding sequence; TEI, translatome enrichment index.
a
Only genes with both UTRs meeting size criterion (see Materials and Methods for details) and with TEI in at least one tissue were considered for calculation of mean and medium values.