Table 1. Conservation and phenotypic consequences of novel DAF-2 mutations.

For each allele, the genotype, residue change (Change), degree of conservation (Cons), amino acid property change (Effect), and predicted phenotypic effect (Prediction) are indicated. In addition, high-temperature induction of dauer (Hid) phenotype, percent dauer (%D), standard deviation (STDEV), standard error (SE), number of worms assayed (N), and number of independent isolates (I.I.) are shown. Residue numbering refers to the preproreceptor.

Colors are shown as defined in Figure 3. WT, Wild Type; I., identical; Ch. Rev., charge reversal.

^aSubstituting residue is found at the corresponding location in a human insulin-like receptor.

^bSubstituting residue maintains conserved amino acid charge.

^cIdentical residue found in 22/27 aligned sequences (File S1).

^dConserved charge change.

^eSubstituting residue maintains hydrophobicity of the transmembrane domain.