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. 2016 Oct 28;7(1):87–93. doi: 10.1534/g3.116.036723

Figure 1.

Figure 1

Schematic representation of the lbk gene, positions of gRNAs, and knock-in cassette. We selected two guides: one at the N-terminus between two putative start sites on the antisense strand (bottom), gRNA-lbk1, and the other overlapping the termination codon on the sense strand (top), gRNA-lbk2. The gRNA sequences are shown in black with PAM sites in red, while gray indicates the complementary sequence. The underline in gRNA-lbk2 indicates the termination codon. The knock-in cassette consisted of a 225 bp 3xFLAG-3XHA flanked by 387 and 384 bp lbk homology arms. The length of genomic lbk from the first putative ATG start codon to the TGA stop codon is 4629 bp.