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. 2016 Nov 1;7(1):95–108. doi: 10.1534/g3.116.035675

Figure 3.

Figure 3

Expression of polyQ does not affect the transcriptome of C. albicans. (A) C. albicans wild-type (WT) and strains harboring 103Q or 230Q expression constructs were grown at 30° (no heat shock [HS]) or subjected to a 10 min 30–42° HS, in the absence (−) and presence (+) of 50 μg/ml doxycycline (dox) to induce polyQ expression; transcript levels of target genes were measured and normalized to the ACT1 loading control. Data represent mean values ± SD from two independent biological replicates. (B) Heat map illustrating the Pearson correlation of RNA sequencing (RNA-seq) global gene expression between WT and strains harboring 103Q or 230Q expression constructs in the absence and presence of 50 μg/ml dox to induce polyQ expression.