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The CD spectra of HSA-control, HSA + Glc, HAS + AA and HSA + AA + Glc in 50 mM sodium phosphate buffer (pH 7.4), 1 mM EDTA and 0.1 mM sodium azide, incubated at 37 °C for 21 days. The arrow shows an increase in the order of mean residue ellipticity (deg cm2 mol−1).