Fig. 2.

Brachypodium leaf width and distance between veins are affected by Pcz. a Leaves of seedlings undergoing mock or Pcz (50 μM) treatments were measured and dissected in the middle. b Safranin O-stained leaf sections were used to count and observe veins under the microscope. c The vein density values were obtained by dividing the total number of veins in the section by the width of the leaf. d The distance between veins was measured using ImageJ software. Scale bar = 0.5 mm. The graphs represent average value (n > 5) and error bars standard deviation. Significant differences among treatments were determined by Student’s t-test. *, P < 0.05