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. 2017 Jan 6;17:5. doi: 10.1186/s12870-016-0965-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Feedback regulation of Brachypodium BR biosynthetic genes after Pcz treatments. a Semi-quantitative RT-PCR of putative BR biosynthetic genes BdDWF4, BdBR6OX2, and BdCPD and the GA biosynthetic gene BdGA20ox after treatment with the indicated concentrations of Pcz and a mock control. BdACT7 is used as an internal loading control. b-d Quantitative real-time RT-PCR analysis evaluating the expression of the same genes under just mock or 50 μM Pcz