Fig. 5.

Phenotypes of the BdDWF4ox and BdBIN2ox transgenic lines, suggesting the involvement of BdDWF4 in BR biosynthesis and BdBIN2 in responses, respectively. a Morphology of three independent BdDWF4 overexpression lines (#5, #1, and #9) along with wild type Col-0 and gul3-D BR overproducing mutant. b Phenotypes of the wild type Col-0 and BdBIN2 overexpressing lines. Line #2 represent mild phenotype, while #3 are examples of severe dwarfism. Scale bar = 2 cms. c-d Quantitative real-time analysis evaluating the heterologous expression of BdDWF4 and BdBIN2 respectively. Line #5 c and #1 (d) which don’t show distinctive phenotype from wild type were used to compare expression of the rest of the lines. AtUBQ10 was used as internal loading control