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. 2017 Jan 5;18:19. doi: 10.1186/s12864-016-3407-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Setup for the in vitro evolution experiment. Each day two single colonies were transferred to 20 mL LB broth, to grow for 8 h. 1 μL of culture was plated on LB plate for overnight growth. This continued for 8 days until 128 tubes of culture was obtained, and 255 samples had been saved for DNA sequencing