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. 2017 Jan 6;48:1. doi: 10.1186/s13567-016-0406-1

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of APPV infected PK15 cells. A porcine anti APPV serum purified by NS3 affinity chromatography and a goat-anti swine Cy3 labelled polyclonal antibody was used for fluorescence staining. Cy3 fluorescence, brightfield and merge images are shown for uninfected and infected cells at 10× magnification. To resolve the perinuclear staining pattern, a cluster of positive cells is also shown at 40× magnification.