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. 2017 Jan 5;15:2. doi: 10.1186/s12958-016-0224-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Evaluation of pErk1/2 and pCreb activation upon LH and hCG treatment. a Mouse Leydig cells were stimulated by increasing doses of LH and hCG. The phosphorylation of Erk1/2 and Creb was evaluated after 15 min by Western blotting (image representative of five independent experiments). b, c Densitometric analysis of pErk1/2 (b) and pCreb (c) signals. The values were normalized over total ERK and represented as means ± SEM, then statistically evaluated (§ = significant vs control; * = significant LH vs hCG; two-way ANOVA and Bonferroni post-tests; P < 0.05; n = 5)