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. 2017 Jan 5;15:2. doi: 10.1186/s12958-016-0224-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Analysis of LH/hCG-induced ERK and CREB phosphorylation in the presence/absence of specific inhibitors. Murine primary Leydig cells were stimulated 15 min by EC₈₀ LH or hCG, in the presence or in the absence of PKA and MEK inhibitors (H-89 and U0126, respectively). pERK and pCREB levels were evaluated by Western blotting (image representative of three independent experiments)