Fig. 6.

Evaluation of testosterone production upon LH or hCG stimulation in the presence or absence of specific inhibitors, in mouse Leydig cells. The cells were stimulated 24 h by EC₈₀ LH and hCG, in the presence or absence of H-89 or U0126. Total testosterone levels were measured by immune-assay and represented as means ± SEM (§ = significant vs “unstimulated, no inhibitors”; * = significant LH/hCG vs corresponding LH/hCG samples without inhibitor; Mann-Whitney’s U-test; P < 0.05; n = 4)