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. 2016 Mar 15;4(1):12. doi: 10.3390/proteomes4010012

Figure 2.

Figure 2

Mass spectrometry of deuterium-labelled peptides. Peptides were resolved as series of mass isotopomers (m0, m1, m2, ...) using matrix-assisted laser desorption ionisation mass spectrometry (MALDI-MS). Experimental mass spectra (red traces) from peptide DGFIDKNDLR (residues 41–50 of slow/ventricular myosin regulatory light chain; MLRV) are displayed from samples taken after 0, 4, 7 or 14 days of deuterium oxide (2H2O) administration in vivo. The blue trace represents the distribution of mass isotopomers predicted from the elemental composition of the peptide and the natural abundances of 12C, 1H, 14N and 16O (mMass software). The m0 (monoisotopic) peak is composed entirely of primary isotopes (i.e., 12C, 1H,14N and 16O), whereas m1, m2, m3 contain either 1, 2 or 3 “heavy“ isotopes (e.g., 13C, 2H, etc.).