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Migration of marker proteins upon sucrose gradient centrifugation. The synaptic vesicle proteins 2 (SV2, 80/86 kDa) and synaptotagmin-1 (Syt-1, 65 kDa), and the amyloid precursor protein (APP, 110 kDA), and Na+/K+-ATPase (NKA, 110 kDa) served as markers for the presynaptic active zone. PM, presynaptic plasma membrane; SV, synaptic vesicles. Six microliters of the respective fractions were applied per lane.
