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. 2015 Aug 19;3(3):249–265. doi: 10.3390/proteomes3030249

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Confirmation of carbonylation on serum albumin and apolipoprotein A1. C57BL/6J mice were sham irradiated (control) or exposed to 17 Gy thoracic irradiation. Lung cell lysates were obtained 24 h postirradiation from three animals per group and DNP-labeled for immunoprecipitation with the OxyBlot antibody. Immunoprecipitated proteins were then Western blotted for (A) serum albumin or (B) apolipoprotein A1. Bar indicates average of band densitometry of three animals per condition; * indicates p < 0.05.