Table 2.
List of top-scored non-redundant (nr) protein matches of the lotus embryo 1-D shotgun mass spectroscopy results, as matched to Green Plant proteome database (SwissProt 57.0).
| Fractions 1 | Protein Accession | Protein Description | Similar 2 | Score 3 | Cover (%) | Peptide sequences | Sig. Peptide Number | Func. Cat. 4 |
|---|---|---|---|---|---|---|---|---|
| 6,(5,2,1,7,3) | ENO1_HEVBR | Enolase 1 OS = Hevea brasiliensis | 19 | 1125 | 42 | TAIAK, YNQLLR, LTSEIGEK, DGGSDYLGK, AGWGVMASHR, MGAEVYHHLK, DGGSDYLGKGVSK, VQIVGDDLLVTNPK, VNQIGSVTESIEAVK, EAMKMGAEVYHHLK, AAVPSGASTGIYEALELR, LAMQEFMILPVGASSFK, SGETEDTFIADLSVGLATGQIK, YGQDATNVGDEGGFAPNIQENK, KYGQDATNVGDEGGFAPNIQENK, YGQDATNVGDEGGFAPNIQENKEGLELLK | 16 | II |
| 4,(1,2,3,5) | HSP7C_PETHY | Heat shock cognate 70 kDa protein OS = Petunia hybrida | 33 | 922 | 33.6 | IEEVD, DISGNPR, NTTIPTKK, ITITNDKGR, DAGVIAGLNVMR, MVNHFVQEFK, NALENYAYNMR, MVNHFVQEFKR, TTPSYVGFTDTER, ARFEELNMDLFR, IINEPTAAAIAYGLDK, NQVAMNPINTVFDAK ATAGDTHLGGEDFDNR, NQVAMNPINTVFDAK, NAVVTVPAYFNDSQR, EQVFSTYSDNQPGVLIQVYEGER | 16 | IX |
| 1,2,3,4,5,6,7 | ACT_GOSHI | Actin OS = Gossypium hirsutum | 14 | 903 | 50.7 | DLTDALMK, AGFAGDDAPR, IKVVAPPER, GYSFTTTAER, HTGVMVGMGQK, EITALAPSSMK, DAYVGDEAQSK, AVFPSIVGRPR, IWHHTFYNELR, LDLAGRDLTDALMK, GYSFTTTAEREIVR, SYELPDGQVITIGAER, VAPEEHPVLLTEAPLNPK, VAPEEHPVLLTEAPLNPK, TTGIVLDSGDGVSHTVPIYEGYALPHAILR | 15 | VII |
| 6,(1,2,3,7) | ACT12_SOLTU | Actin-100 (Fragment) OS = Solanum tuberosum | 5 | 872 | 53.5 | AGFAGDDAPR, IKVVAPPER, HTGVMVGMGQK, EITALAPSSMK, DAYVGDEAQSK, AVFPSIVGRPR, DAYVGDEAQSKR, GEYDESGPSIVHR, IWHHTFYNELR, SYELPDGQVITIGAER, LAYVALDYEQELETAK, YPIEHGIVSNWDDMEK, TTGIVLDSGDGVSHTVPIYEGYALPHAILR | 13 | VII |
| 7,(8,2) | G3PC_ANTMA | Glyceraldehyde-3-phosphate dehydrogenase, cytosolic OS = Antirrhinum majus | 20 | 749 | 43.6 | VALQR, SSIFDAK, KATYEQIK, AAIKEESEGK, AGIALNDNFVK, DAPMFVVGVNEK, AASFNIIPSSTGAAK, VPTVDVSVVDLTVR, VPTVDVSVVDLTVRLEK, FGIVEGLMTTVHSITATQK, GILGYTEDDVVSTDFVGDSR, LTGMSFRVPTVDVSVVDLTVR, LKGILGYTEDDVVSTDFVGDSR, VINDRFGIVEGLMTTVHSITATQK | 14 | II |
| 4,(1,5,2) | HSP83_IPONI | Heat shock protein 83 OS = Ipomoea nil | 4 | 717 | 31 | VIVTTK, VVVSDR, KLVSATK, AILFVPK, EMLQQNK, DVDGEQLGR, FESLTDKSK, APFDLFDTR, AVENSPFLER, LGIHEDSQNR, DIYYITGESK, LDAQPELFIR, RAPFDLFDTR, ADLVNNLGTIAR, ELISNASDALDK, KAVENSPFLER, HFSVEGQLEFK, GVVDSDDLPLNISR, ELISNASDALDKIR, SGDELTSLKDYVTR, KPEEITKEEYASFYK, HSEFISYPIYLWTEK, ITLFLKEDQLEYLEER | 23 | IX |
| 6 | ATPBM_MAIZE | ATP synthase subunit beta, mitochondrial OS = Zea mays | 3 | 712 | 31.8 | IGLFGGAGVGK, VVDLLAPYQR, TIAMDGTEGLVR, AHGGFSVFAGVGER, VGLTGLTVAEHFR, VLNTGSPITVPVGR, TVLIMELINNVAK, FTQANSEVSALLGR, QISELGIYPAVDPLDSTSR, EAPAFVEQATEQQILVTGIK, IPSAVGYQPTLATDLGGLQER | 11 | I |
| 4,(5,2,1,3) | HSP7E_SPIOL | Chloroplast envelope membrane 70 kDa heat shock-related protein OS = Spinacia oleracea | 5 | 631 | 31.2 | NTTIPTKK, LSKEEIEK, TRDNNLLGK, DAGVISGLNVMR, EIAEAYLGSTVK, NALENYAYNMR, TTPSYVAFTDSER, IINEPTAAAIAYGLDK, ATAGDTHLGGEDFDNR, NQVAMNPINTVFDAK, NAVVTVPAYFNDSQR, EQVFSTYSDNQPGVLIQVYEGER | 12 | IX |
| 4,(2,1,5) | HSP81_ORYSI | Heat shock protein 81-1 OS = Oryza sativa subsp. Indica | 9 | 610 | 35.1 | NLVKK, VVVTTK, IAELLR, KLVSATK, EMLQQNK, FESLTDKSK, APFDLFDTR, DSSMAGYMSSK, RAPFDLFDTR, KAVENSPFLEK, SDLVNNLGTIAR, HFSVEGQLEFK, EVSHEWSLVNK, GIVDSEDLPLNISR, SLTNDWEEHLAVK, SGDELTSLKDYVTR, LDAQPELFIHIVPDK, HSEFISYPISLWTEK, KPEEITKEEYAAFYK, MKEGQNDIYYITGESK, KHSEFISYPISLWTEK | 21 | IX |
| 6,(3,1,2) | TBB_HORVU | Tubulin beta chain OS = Hordeum vulgare | 21 | 583 | 39.1 | YLTASAMFR, IREEYPDR, LAVNLIPFPR, VSEQFTAMFR, YTGTSDLQLER, MMLTFSVFPSPK, EVDEQMINVQNK, LHFFMVGFAPLTSR, AVLMDLEPGTMDSVR, LHFFMVGFAPLTSR, NSSYFVEWIPNNVK, ALTVPELTQQMWDAK, GHYTEGAELIDSVLDVVRK, TGPYGQIFRPDNFVFGQSGAGNNWAK | 14 | VII |
| 6,(1,7,3,4,2) | EF1A_TOBAC | Elongation factor 1-alpha OS = Nicotiana tabacum | 17 | 574 | 38.5 | YDEIVK, GFVASNSK, EVSSYLK, QTVAVGVIK, EVSSYLKK, RGFVASNSK, LPLQDVYK, ARYDEIVK, IGGIGTVPVGR, STNLDWYK, STTTGHLIYK, EHALLAFTLGVK, GFVASNSKDDPAK, YYCTVIDAPGHR, YDEIVKEVSSYLK, YYCTVIDAPGHRDFIK, MIPTKPMVVETFSEYPPLGR, NMITGTSQADCAVLIIDSTTGGFEAGISK | 18 | V |
| 4,(1,2,5,4) | HS901_ARATH | Heat shock protein 90-1 OS = Arabidopsis thaliana | 6 | 539 | 26.4 | VVVTTKVVVTTK, VVVSDR, KLVSATK, AILFVPK, FESLTDKSK, APFDLFDTR, AVENSPFLER, LGIHEDSQNR, DSSMSGYMSSK, RAPFDLFDTR, ADLVNNLGTIAR, KAVENSPFLER, HFSVEGQLEFK, TLSIIDSGIGMTK, GVVDSDDLPLNISR, KPEEITKEEYAAFYK, HSEFISYPIYLWTEK | 17 | IX |
| 4,(2,1,3) | METE_ARATH | 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase OS = Arabidopsis thaliana | 12 | 536 | 20.9 | AAAALK, VVEVNALAK, SWLAFAAQK, AVNEYKEAK, YLFAGVVDGR, SDEKLLSVFR, FALESFWDGK, GNASVPAMEMTK, YGAGIGPGVYDIHSPR, GMLTGPVTILNWSFVR | 10 | I |
| 4 | ENPL_CATRO | Endoplasmin homolog OS = Catharanthus roseus | 3 | 472 | 12.6 | NLGTIAK, FWNEFGK, YGWSSNMER, ELISNASDALDK, IMQSQTLSDASK, GLVDSDTLPLNVSR, ELISNASDALDKIR, VFISDEFDELLPK, RVFISDEFDELLPK, LMDIIINSLYSNKDIFLR | 10 | IX |
| 4,(5,2) | BIP4_TOBAC | Luminal-binding protein 4 OS = Nicotiana tabacum | 6 | 454 | 21 | LIGEAAK, NTVIPTKK, IMEYFIK, LSQEEIER, ITITNDKGR, ALSSQHQVR, EAEEFAEEDKK, IVNKDGKPYIQVK, ARFEELNNDLFR, IINEPTAAAIAYGLDK, IKDAVVTVPAYFNDAQR | 11 | IX |
| 4,(1,3,2,7) | EF2_BETVU | Elongation factor 2 OS = Beta vulgaris | 6 | 454 | 16.6 | DLYVK, VASDLPK, GGGQIIPTAR, MIPASDKGR, IRPVLTVNK, EGALAEENMR, NMSVIAHVDHGK, FGVDESKMMER, VFYASQLTAKPR, LWGENFFDPATK, IRPVLTVNKMDR, RVFYASQLTAKPR, GHVFEEMQRPGTPLYNIK, RGHVFEEMQRPGTPLYNIK | 15 | V |
| 4,(1,2,3) | HSP82_MAIZE | Heat shock protein 82 OS = Zea mays | 5 | 429 | 16.1 | VVVSDR, KLVSATK, APFDLFDTR, AVENSPFLER, LGIHEDSQNR, RAPFDLFDTR, SDLVNNLGTIAR, ELISNASDALDK, KAVENSPFLER, HFSVEGQLEFK, GVVDSDDLPLNISR, ELISNASDALDKIR, HSEFISYPIYLWTEK | 13 | IX |
| 6,(2,3) | IF4A1_ORYSJ | Eukaryotic initiation factor 4A-1 OS = Oryza sativa subsp. Japonica | 4 | 418 | 31.4 | ALGDYLGVK, ELAQQIEK, KGVAINFVTR, VLITTDLLAR, QSLRPDYIK, RDELTLEGIK, GLDVIQQAQSGTGK, GIYAYGFEKPSAIQQR, GFKDQIYDIFQLLPSK | 9 | V |
| 3,(2,1) | CAPPC_FLATR | Phosphoenolpyruvate carboxylase 2 OS = Flaveria trinervia | 32 | 417 | 18 | GIAAGMQNTG, MNIGSRPSK, VILGDVRDK, KPSGGIESLR, LSAAWQLYK, SPEEVFDALK, RPLFGPDLPK, TPPTPQDEMR, QVSTFGLSLVR, VTIDLVEMVFAK, AGMSYFHETIWK, AIPWIFAWTQTR, VPYNAPLIQFSSWMGGDRDGNPR | 13 | I |
| 6,(2) | ENO2_ARATH | Bifunctional enolase 2/transcriptional activator OS = Arabidopsis thaliana | 2 | 391 | 33.3 | YNQLLR, DGGSDYLGK, ISGDALKDLYK, DGGSDYLGKGVSK, VNQIGSVTESIEAVK, IVLPVPAFNVINGGSHAGNK, SGETEDTFIADLAVGLSTGQIK, YGQDATNVGDEGGFAPNIQENK, KYGQDATNVGDEGGFAPNIQENK, YGQDATNVGDEGGFAPNIQENKEGLELLK | 10 | IV |
| 5,(2) | CH61_CUCMA | Chaperonin CPN60-1, mitochondrial OS = Cucurbita maxima | 6 | 387 | 33.33 | ISSINAVVK, VTDALNATK, VTKDGVTVAK, KISSINAVVK, IGGASEAEVGEK, IGVQIIQNALK, IGGASEAEVGEKK, GYISPYFITNQK, AAVEEGIVPGGGVALLYASK, TPVHTIASNAGVEGAVVVGK | 10 | IX |
| 4 | HSP7L_ARATH | Heat shock 70 kDa protein 12 OS = Arabidopsis thaliana | 1 | 382 | 16.8 | NTVIPTKK, IMEYFIK, ALSSQHQVR, EAEEFAEEDKK, ARFEELNNDLFR, ARFEELNNDLFR, IINEPTAAAIAYGLDK, IKDAVVTVPAYFNDAQR | 8 | IX |
| 2,(1) | CLAH1_ARATH | Clathrin heavy chain 1 OS = Arabidopsis thaliana | 4 | 374 | 10.3 | TVDNDLALK, SPEQVSAAVK, VANVELYYK, DPTLAVVAYR, FQELFAQTK, VEEDAVWSQVAK, GNLPGAENLVVQR, EGLVSDAIESFIR, GNMQLFSVDQQR, KNLLENWLAEDK, RGNLPGAENLVVQR, QLIDQVVSTALPESK, YKEAAELAAESPQGILR | 13 | VI |
| 6,(2,5,1) | ATPAM_PEA | ATP synthase subunit alpha, mitochondrial OS = Pisum sativum | 1 | 364 | 29.4 | VVSVGDGIAR, TGSIVDVPAGK, AAELTTLLESR, VVDALGVPIDGR, TAIAIDTILNQK, KSVHEPMQTGLK, GIRPAINVGLSVSR, EAFPGDVFYLHSR, ITNFYTNFQVDEIGR, LTEVLKQPQYAPLPIEK, EVAAFAQFGSDLDAATQALLNR | 11 | I |
| 5 | RUBB_PEA | RuBisCO large subunit-binding protein subunit beta, chloroplastic OS = Pisum sativum | 2 | 357 | 23.98 | IAALK, VVLTK, NVVLESK, VEDALNATK, IVNDGVTVAK, KGVVTLEEGK, LADLVGVTLGPK, GYISPYFVTDSEK, EVELEDPVENIGAK, TNDLAGDGTTTSVVLAQGLIAEGVK, IVNDGVTVAKEVELEDPVENIGAK | 11 | I |
| 5,(2) | PGMC_PEA | Phosphoglucomutase, cytoplasmic OS = Pisum sativum | 5 | 333 | 41.16 | YLFEDGSR, FFEVPTGWK, LSGTGSEGATIR, SMPTSAALDVVAK, YDYENVDAGAAK | 5 | II |
| 6,(2) | SAHH_MESCR | Adenosylhomocysteinase OS = Mesembryanthemum crystallinum | 6 | 330 | 11.1 | ATDVMIAGK, HSLPDGLMR, ITIKPQTDR, TEFGPSQPFK, LVGVSEETTTGVK, TEFGPSQPFKGAK, LVGVSEETTTGVKR | 7 | I |
| 7 | PGKY_TOBAC | Phosphoglycerate kinase, cytosolic OS = Nicotiana tabacum | 7 | 302 | 21.7 | LAELSGK, YSLKPLVPR, YLKPAVAGFLMQK, GVSLLLPTDVVIADK, GVTTIIGGGDSVAAVEK, LASLADLYVNDAFGTAHR, KLASLADLYVNDAFGTAHR | 7 | II |
| 4 | SUSY_SOYBN | Sucrose synthase OS = Glycine max | 12 | 295 | 13.4 | YLEMFYALK, VVHGIDVFDPK, NITGLVEWYGK, ELVNLVVVAGDR, LLPDAVGTTCGQR, SGFHIDPYHGDR, LGVTQCTIAHALEK | 7 | II |
| 5 | CPNB3_ARATH | Chaperonin 60 subunit beta 3, chloroplastic OS = Arabidopsis thaliana | 1 | 292 | 25.35 | VVLTK, NVVLESK, VEDALNATK, KGVVTLEEGK, LADLVGVTLGPK, GYISPYFVTDSEK, EVELEDPVENIGAK, TNDLAGDGTTTSVVLAQGLIAEGVK | 8 | IX |
| 7,(8) | MDHC2_ARATH | Malate dehydrogenase, cytoplasmic 2 OS = Arabidopsis thaliana | 2 | 288 | 23.2 | GAAIIK, NVSIYK, SQASALEK, EFAPSIPEK, MELVDAAFPLLK, VLVVANPANTNALILK, VLVTGAAGQIGYALVPMIAR | 7 | II |
| 8,(1) | 1433E_TOBAC | 14-3-3-like protein E OS = Nicotiana tabacum | 19 | 284 | 29.8 | NVIGAR, VFYLK, YLAEFK, MKGDYHR, NLLSVAYK, IISSIEQK, TVDVEELTVEER, IISSIEQKEESR, SAQDIALAELAPTHPIR | 9 | VIII |
| 5 | VATA_GOSHI | V-type proton ATPase catalytic subunit A OS = Gossypium hirsutum | 2 | 272 | 49.1 | SGDVYIPR, TVISQALSK, LAADTPLLTGQR, LAEMPADSGYPAYLAAR, LTTFEDSEKESEYGYVR, LVSQKFEDPAEGEEALVAK | 6 | VI |
| 4,(3,2,1) | CD48A_ARATH | Cell division control protein 48 homolog A OS = Arabidopsis thaliana | 3 | 265 | 18.5 | TLLAK, KGDLFLVR, RSVSDADIR, DFSTAILER, LAEDVDLER, GILLYGPPGSGK, LAGESESNLRK, IVSQLLTLMDGLK, ELVELPLRHPQLFK, NAPSIIFIDEIDSIAPK | 10 | III/IV |
| 7 | ALF_CICAR | Fructose-bisphosphate aldolase, cytoplasmic isozyme OS = Cicer arietinum | 1 | 245 | 11.1 | GILAADESTGTIGK, GILAADESTGTIGKR, YHDELIANAAYIGTPGK | 3 | II |
| 7 | MDHM_CITLA | Malate dehydrogenase, mitochondrial OS = Citrullus lanatus | 2 | 235 | 17.6 | LFGVTTLDVVR, TQDGGTEVVEAK, DDLFNINAGIVK, RTQDGGTEVVEAK, VAVLGAAGGIGQPLALLMK | 5 | II |
| 6,7 | ACT5_ARATH | Putative actin-5 OS = Arabidopsis thaliana | 1 | 213 | 20.4 | AGFAGDDAPR, IKVVAPPER, IWHHTFYNELR, TTGIVLDSGDGVSHTVPIYEGYALPHAILR | 4 | VII |
| 6 | UGPA_MUSAC | UTP--glucose-1-phosphate uridylyltransferase OS = Musa acuminata | 3 | 199 | 18 | VANFLSR, GGTLISYEGR, VLQLETAAGAAIR, FFDHAIGINVPR, LQSAVAELNQISENEK | 5 | II |
| 8 | ADT1_GOSHI | ADP, ATP carrier protein 1, mitochondrial OS = Gossypium hirsutum | 2 | 194 | 8.8 | SSLDAFSQILK, LLIQNQDEMIK, YFPTQALNFAFK | 3 | VI |
| 8 | RAN_VICFA | GTP-binding nuclear protein Ran/TC4 OS = Vicia faba | 1 | 193 | 26.2 | NVPTWHR, HLTGEFEK, AKQVTFHR, LVIVGDGGTGK, NLQYYEISAK, SNYNFEKPFLYLAR | 6 | VIII |
| 3 | CLPB1_ARATH | Chaperone protein ClpB1 OS = Arabidopsis thaliana | 2 | 192 | 11.1 | TAVVEGLAQR, YRGEFEER, TKNNPVLIGEPGVGK, KVESASGDTNFQALK, VQLDSQPEEIDNLER, LIGAPPGYVGHEEGGQLTEAVR | 6 | IX |
| 5 | CPNA1_ARATH | Chaperonin 60 subunit alpha 1, chloroplastic OS = Arabidopsis thaliana | 1 | 189 | 82.82 | VVNDGVTIAR, NVVLDEFGSPK, VGAATETELEDR | 3 | IX |
| 3 | ACOC_CUCMA | Aconitate hydratase, cytoplasmic OS = Cucurbita maxima | 6 | 187 | 9.2 | NFEGR, ILLESAIR, STYESITK, DFNSYGSR, RGNDEVMAR, TSLAPGSGVVTK, ATIANMSPEYGATMGFFPVDHVTLQYLK | 7 | II |
| 3 | SYA_ARATH | Alanine--tRNA ligase OS = Arabidopsis thaliana | 1 | 181 | 5.4 | LTSVLQNK, HVDTGMGFER, ESDGSLKPLPAK, AFALLSEEGIAK, AVFGEVYPDPVR | 5 | IV |
| 6,(7) | PRS6A_SOLLC | 26S protease regulatory subunit 6A homolog OS = Solanum lycopersicum | 4 | 170 | 8.5 | IIKEELQR, GVLLYGPPGTGK, LAGPQLVQMFIGDGAK | 3 | I |
| 7 | AATM_LUPAN | Aspartate aminotransferase P2, mitochondrial (Fragment) OS = Lupinus angustifolius | 1 | 170 | 10.8 | IADVIQEK, NLGLYAER, LNLGVGAYR, ISLAGLSLAK, VATVQGLSGTGSLR | 5 | I |
| 6 | UGDH_SOYBN | UDP-glucose 6-dehydrogenase OS = Glycine max | 1 | 168 | 6.5 | IAILGFAFK, LAANAFLAQR, AADLTYWESAAR | 3 | II |
| 2,8 | ANX4_FRAAN | Annexin-like protein RJ4 OS = Fragaria ananassa | 1 | 163 | 9.6 | VGTDEDALTR, LLVALVTAYR | 2 | IX |
| 8 | RS4_GOSHI | 40S ribosomal protein S4 OS = Gossypium hirsutum | 4 | 163 | 22.9 | LSIIEEAR, LGNVFTIGK, GIPYLNTYDGR, LGGAFAPKPSSGPHK, TDKTYPAGFMDVVSIPK | 5 | V |
| 7,(8) | RSSA_SOYBN | 40S ribosomal protein SA OS = Glycine max | 2 | 161 | 19 | LLILTDPR, YVDIGIPANNK, HTPGTFTNQLQTSFSEPR, VIVAIENPQDIIVQSARPYGQR | 4 | V |
| 8 | RAA1D_ARATH | Ras-related protein RABA1d OS = Arabidopsis thaliana | 9 | 161 | 26.6 | AITSAYYR, VVLIGDSGVGK, STIGVEFATR, HSTFENVER, AQIWDTAGQER | 5 | VIII |
| 8 | RS18_ARATH | 40S ribosomal protein S18 OS = Arabidopsis thaliana | 1 | 155 | 23.7 | LRDDLER, VLNTNVDGK, IPDWFLNR, YSQVVSNALDMK | 4 | V |
| 1 | AVP_VIGRR | Pyrophosphate-energized vacuolar membrane proton pump OS = Vigna radiata var. Radiata | 1 | 151 | 9.5 | TDALDAAGNTTAAIGK, AAVIGDTIGDPLKDTSGPSLNILIK | 2 | VI |
| 5 | ILV5_ARATH | Ketol-acid reductoisomerase, chloroplastic OS = Arabidopsis thaliana | 1 | 150 | 27.45 | SDIVVK, SVVLAGR, QIGVIGWGSQGPAQAQNLR | 3 | I |
| 7 | AATC_DAUCA | Aspartate aminotransferase, cytoplasmic OS = Daucus carota | 2 | 149 | 10.4 | ISMAGLSSR, LNLGVGAYR, LIFGADSPAIQENR | 3 | I |
| 8 | RL13_TOBAC | 60S ribosomal protein L13 OS = Nicotiana tabacum | 1 | 149 | 23.3 | GFSLEELK, TWFNQPAR, SLEGLQTNVQR, KLAPTIGIAVDHR | 4 | V |
| 5 | ACLB1_ORYSJ | ATP-citrate synthase beta chain protein 1 OS = Oryza sativa subsp. Japonica | 1 | 148 | 35.6 | FNNIPQVK, FGGAIDDAAR, SEVQFGHAGAK, SIGLIGHTFDQKR, VVAIIAEGVPESDTK | 5 | I |
| 2,(3) | COPA1_ARATH | Coatomer subunit alpha-1 OS = Arabidopsis thaliana | 3 | 147 | 4.4 | VWDIGALR, YVLEGHDR, AWEVDTLR, VVIFDLQQR, TLDVPIYITK, QDIIVSNSEDK | 6 | VI |
| 6 | CATA2_RICCO | Catalase isozyme 2 OS = Ricinus communis | 2 | 146 | 9.3 | FSTVIHER, APGVQTPVIVR, EGNFDIVGNNFPVFFIR | 3 | IX |
| 8 | RAN3_ORYSI | GTP-binding nuclear protein Ran-3 OS = Oryza sativa subsp. Indica | 1 | 145 | 14.2 | HITGEFEK, NLQYYEISAK, SNYNFEKPFLYLAR | 3 | III/VI |
| 6 | MDAR_SOLLC | Monodehydroascorbate reductase OS = Solanum lycopersicum | 1 | 139 | 12.5 | AYLFPEGAAR, LSDFGVQGADSK, IVGAFLESGSPEENKAIAK | 3 | IX |
| 6,(1) | RL3_ORYSJ | 60S ribosomal protein L3 OS = Oryza sativa subsp. Japonica | 4 | 138 | 9.3 | VIAHTQIR, HGSLGFLPR, LALEEIKLK, GKGYEGVVTR | 4 | V |
| 5 | PMGI_RICCO | 2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS = Ricinus communis | 4 | 136 | 43.5 | ARDAILSGK, LVDLALASGK, LDQLQLLLK, AHGTAVGLPTEDDMGNSEVGHNALGAGR | 4 | II |
| 5 | RUBA_RICCO | RuBisCO large subunit-binding protein subunit alpha (Fragment) OS = Ricinus communis | 2 | 134 | 71.19 | NVVLDEFGSPK, VGAATETELEDR, LGLLSVTSGANPVSIK | 3 | I |
| 7 | RL4_PRUAR | 60S ribosomal protein L4 OS = Prunus armeniaca | 1 | 134 | 14.5 | AGHQTSAESWGTGR, YAVVSAIAASAVPSLVLAR | 2 | V |
| 5 | G6PI_SPIOL | Glucose-6-phosphate isomerase, cytosolic OS = Spinacia oleracea | 2 | 131 | 27 | SQQPVYLK, FLANVDPIDVAK, TFTTAETMLNAR | 3 | II |
| 8 | RS8_MAIZE | 40S ribosomal protein S8 OS = Zea mays | 1 | 130 | 21.7 | LDTGNYSWGSEAVTR, ILDVVYNASNNELVR | 2 | V |
| 8 | RL11_MEDSA | 60S ribosomal protein L11 OS = Medicago sativa | 1 | 129 | 17.7 | YEGVILNK, AMQLLESGLK, VLEQLSGQTPVFSK | 3 | V |
| 8,(7) | H4_ARATH | Histone H4 OS = Arabidopsis thaliana | 2 | 128 | 46.6 | TLYGFGG, IFLENVIR, DAVTYTEHAR, ISGLIYEETR | 4 | VII |
| 5 | TCPE_ARATH | T-complex protein 1 subunit epsilon OS = Arabidopsis thaliana | 1 | 123 | 73.41 | IAEGYEMASR, QQQILLATQVVK | 2 | V |
| 5 | TCPA_ARATH | T-complex protein 1 subunit alpha OS = Arabidopsis thaliana | 1 | 119 | 71.23 | YFVEAGAIAVR, NKIHPTSIISGYR | 2 | V |
| 4 | TKTC_SPIOL | Transketolase, chloroplastic OS = Spinacia oleracea | 4 | 118 | 6.1 | FLAIDAVEK, ALPTYTPETPGDATR, VIPGLLGGSADLASSNMTLLK | 3 | II |
| 8 | TPIS_MAIZE | Triosephosphate isomerase, cytosolic OS = Zea mays | 1 | 117 | 11.9 | FFVGGNWK, VAYALSQGLK, VIACVGETLEQR | 3 | VII |
| 8 | PROF3_ARATH | Profilin-3 OS = Arabidopsis thaliana | 2 | 117 | 17.2 | LGDYLLEQGL, YMVIQGEPGAVIR | 2 | VII |
| 8 | RS92_ARATH | 40S ribosomal protein S9-2 OS = Arabidopsis thaliana | 1 | 115 | 19.3 | LVGEYGLR, ERLDAELK, RPYEKER, RLQTIVFK, IFEGEALLR | 5 | V |
| 6 | VATB1_ARATH | V-type proton ATPase subunit B1 OS = Arabidopsis thaliana | 1 | 115 | 10.5 | YQEIVNIR, TVSGVAGPLVILDK, QIYPPINVLPSLSR | 3 | VI |
| 6 | ERF1X_ARATH | Eukaryotic peptide chain release factor subunit 1-1 OS = Arabidopsis thaliana | 1 | 113 | 9.9 | GFGGIGGILR, QSVLGAITSAQQR | 2 | V |
| 5 | HSP7M_PHAVU | Heat shock 70 kDa protein, mitochondrial OS = Phaseolus vulgaris | 3 | 111 | 24.87 | HLNITLTR, VIENSEGAR, TTPSVVAFNQK, SSGGLSEDEIEK | 4 | IX |
| 8 | ANXD1_ARATH | Annexin D1 OS = Arabidopsis thaliana | 1 | 110 | 5 | AQINATFNR, SKAQINATFNR | 2 | IX |
| 8 | RS16_FRIAG | 40S ribosomal protein S16 OS = Fritillaria agrestis | 3 | 109 | 13.8 | ALVAYYQK, AFEPILLLGR, YKAFEPILLLGR | 3 | V |
| 8 | RS5_CICAR | 40S ribosomal protein S5 (Fragment) OS = Cicer arietinum | 1 | 108 | 15.7 | IGSAGVVRR, GSSNSYAIK, VNQAIYLLTTGAR | 3 | V |
| 8 | ARF_VIGUN | ADP-ribosylation factor OS = Vigna unguiculata | 1 | 104 | 28.7 | ILMVGLDAAGK, NISFTVWDVGGQDK | 2 | VIII |
| 4 | SYGM1_ARATH | Glycine--tRNA ligase 1, mitochondrial OS = Arabidopsis thaliana | 1 | 103 | 5.1 | LFYIPSFK, VFTPSVIEPSFGIGR | 2 | IV/VI |
| 7 | RGP1_ORYSJ | UDP-arabinopyranose mutase 1 OS = Oryza sativa subsp. Japonica | 1 | 101 | 6.9 | ILGPK, ASNPFVNLK, ASNPFVNLKK, YVDAVMTVPK | 4 | I |
| 3 | HSP7O_ARATH | Heat shock 70 kDa protein 14 OS = Arabidopsis thaliana | 1 | 101 | 5.1 | ILSHAFDR, NAVESYVYDMR, AVLDAATIAGLHPLR | 3 | IX |
| 8 | RS15A_DAUCA | 40S ribosomal protein S15a OS = Daucus carota | 1 | 100 | 29.2 | VSVLNDALK, HGYIGEFEYVDDHR | 2 | V |
| 8 | RS61_ARATH | 40S ribosomal protein S6-1 OS = Arabidopsis thaliana | 2 | 100 | 18 | LVTPLTLQR, KGENDLPGLTDTEKPR, ISQEVSGDALGEEFKGYVFK | 3 | V |
| 6 | ACCC2_POPTR | Biotin carboxylase 2, chloroplastic OS = Populus trichocarpa | 1 | 97 | 7.8 | LLEEAPSPALTPELR, ALDDTVITGVPTTIDYHK | 2 | I |
| 8 | RLA2_PARAR | 60S acidic ribosomal protein P2 OS = Parthenium argentatum | 1 | 97 | 10.5 | DITELIASGR, GKDITELIASGR | 2 | V |
| 8 | RS33_ARATH | 40S ribosomal protein S3-3 OS = Arabidopsis thaliana | 3 | 96 | 10.9 | ELAEDGYSGVEVR, FKFPQDSVELYAEK | 2 | V |
| 5 | KPYC_SOYBN | Pyruvate kinase, cytosolic isozyme OS = Glycine max | 1 | 94 | 55.66 | KGSDLVNVR, STPLPMSPLESLASSAVR | 2 | II |
| 7 | GMD1_ARATH | GDP-mannose 4,6 dehydratase 1 OS = Arabidopsis thaliana | 2 | 93 | 5 | RGENFVTR, LFLGNIQASR | 2 | II |
| 4 | HSP7S_SPIOL | Stromal 70 kDa heat shock-related protein, chloroplastic (Fragment) OS = Spinacia oleracea | 2 | 93 | 5.3 | HIETTLTR, IINEPTAASLAYGFEK | 2 | IX |
| 6 | EF1G2_ORYSJ | Elongation factor 1-gamma 2 OS = Oryza sativa subsp. Japonica | 3 | 93 | 16.7 | EVAIK, LYSNTK, NPLDLLPPSK, MILDEWKR, SFTSEFPHVER | 5 | V |
| 8 | RLA0_LUPLU | 60S acidic ribosomal protein P0 OS = Lupinus luteus | 1 | 90 | 9.9 | EYLKDPSK, VGSSEAALLAK | 2 | V |
| 7 | GCST_PEA | Aminomethyltransferase, mitochondrial OS = Pisum sativum | 1 | 89 | 8.3 | GGAIDDSVITK, TGYTGEDGFEISVPSEHGVELAK | 2 | I |
| 8 | APX1_ORYSJ | L-ascorbate peroxidase 1, cytosolic OS = Oryza sativa subsp. Japonica | 1 | 89 | 15.2 | TGGPFGTMK, LSELGFADA, ALLSDPAFRPLVEK | 3 | IX |
| 8 | RS193_ARATH | 40S ribosomal protein S19-3 OS = Arabidopsis thaliana | 1 | 88 | 23.1 | AYAAHLKR, TVKDVSPHEFVK, ELAPYDPDWYYIR | 3 | V |
| 1,(2) | RPN1A_ARATH | 26S proteasome non-ATPase regulatory subunit 2 1A OS = Arabidopsis thaliana | 3 | 87 | 5.5 | VGQAVDVVGQAGRPK, NLAGEIAQEYTKR | 2 | I |
| 4,(3) | PPDK_FLABR | Pyruvate, phosphate dikinase, chloroplastic OS = Flaveria brownii | 8 | 87 | 2.9 | SDFEGIFR, AALIADEIAK, AMDGLPVTIR | 3 | II |
| 8 | RS13_PEA | 40S ribosomal protein S13 OS = Pisum sativum | 1 | 87 | 25.8 | DSHGIAQVK, GLTPSQIGVILR, KGLTPSQIGVILR, AHGLAPEIPEDLYHLIK | 4 | V |
| 8 | RS14_CHLRE | 40S ribosomal protein S14 OS = Chlamydomonas reinhardtii | 1 | 85 | 18.3 | TPGPGAQSALR, IEDVTPIPTDSTRR | 2 | V |
| 6 | VATB2_GOSHI | V-type proton ATPase subunit B 2 (Fragment) OS = Gossypium hirsutum | 1 | 84 | 10.1 | FVTQGAYDTR, QIYPPINVLPSLSR | 2 | VI |
| 8,(5) | CYPH_MAIZE | Peptidyl-prolyl cis-trans isomerase OS = Zea mays | 3 | 83 | 16.3 | SGKPLHYK, VFFDMTVGGAPAGR | 2 | V |
| 8 | NDK1_ARATH | Nucleoside diphosphate kinase 1 OS = Arabidopsis thaliana | 1 | 82 | 9.4 | NVIHGSDSVESAR, NVIHGSDSVESARK | 2 | I |
| 7 | GLN11_ORYSJ | Glutamine synthetase cytosolic isozyme 1-1 OS = Oryza sativa subsp. Japonica | 1 | 79 | 7.6 | DIVDSHYK, HKEHISAYGEGNER | 2 | I |
| 7 | SERC_SPIOL | Phosphoserine aminotransferase, chloroplastic OS = Spinacia oleracea | 1 | 79 | 5.3 | FGLIYAGAQK, NVGPSGVTIVIVR | 2 | I |
| 7 | PDI21_ARATH | Protein disulfide-isomerase like 2-1 OS = Arabidopsis thaliana | 1 | 78 | 8.9 | KLAPEYEK, YGVSGFPTLK, YGVSGYPTIQWFPK | 3 | V |
| 8 | RS254_ARATH | 40S ribosomal protein S25-4 OS = Arabidopsis thaliana | 2 | 76 | 29.6 | LITPSILSDR, MVAAHSSQQIYTR | 2 | V |
| 6 | IDHC_TOBAC | Isocitrate dehydrogenase [NADP] OS = Nicotiana tabacum | 2 | 75 | 7.5 | HAFGDQYR, DLALIIHGSK, TIEAEAAHGTVTR | 3 | II |
| 6 | OPD22_ARATH | Dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial OS = Arabidopsis thaliana | 1 | 73 | 3.9 | ISVNDLVIK, VIDGAIGAEWLK | 2 | II |
| 8 | RL40A_ARATH | Ubiquitin-60S ribosomal protein L40-1 OS = Arabidopsis thaliana | 1 | 70 | 45.3 | ESTLHLVLR, TITLEVESSDTIDNVK | 2 | V |
| 8 | RL24_PRUAV | 60S ribosomal protein L24 OS = Prunus avium | 1 | 69 | 7 | SIVGATLEVIQK, SIVGATLEVIQKR | 2 | V |
| 7,(5) | SAPK6_ORYSJ | Serine/threonine-protein kinase SAPK6 OS = Oryza sativa subsp. Japonica | 2 | 68 | 7.4 | DIGSGNFGVAR, STVGTPAYIAPEVLSR | 2 | III |
| 6 | GME2_ORYSJ | GDP-mannose 3,5-epimerase 2 OS = Oryza sativa subsp. Japonica | 1 | 67 | 7 | NSDNTLIKEK, ISITGAGGFIASHIAR | 2 | II |
| 8 | EF1D1_ORYSJ | Elongation factor 1-delta 1 OS = Oryza sativa subsp. Japonica | 2 | 66 | 7.9 | LVPVGYGIK, KLDEYLLTR | 2 | V |
| 8 | IF5A1_ARATH | Eukaryotic translation initiation factor 5A-1 OS = Arabidopsis thaliana | 1 | 64 | 12 | VVEVSTSK, TYPQQAGTIR, TYPQQAGTIRK | 3 | V |
| 8 | H2B11_ARATH | Histone H2B.11 OS = Arabidopsis thaliana | 1 | 62 | 30 | LVLPGELAK, QVHPDIGISSK, YNKKPTITSR | 3 | VII |
| 8 | PSA3_ARATH | Proteasome subunit alpha type-3 OS = Arabidopsis thaliana | 1 | 60 | 7.6 | VFQIEYAAK, VPDDLLEEAK | 2 | I |
| 7 | AAT3_ARATH | Aspartate aminotransferase, chloroplastic OS = Arabidopsis thaliana | 1 | 60 | 4.9 | LNLGVGAYR, TEEGKPLVLNVVR | 2 | I |
| 1 | PDR4_ORYSJ | Pleiotropic drug resistance protein 4 OS = Oryza sativa subsp. Japonica | 1 | 60 | 1.5 | TTLLLALAGK, VTTGEMLVGPAR | 2 | IX |
| 2,(8) | UBQ12_ARATH | Polyubiquitin 12 OS = Arabidopsis thaliana | 3 | 60 | 23.9 | MQIFLKTLTGK, IQDKEGIPPDQQR, TITLEVESSDTIDNVK | 3 | V |
| 2,(1) | UBIQ_AVESA | Ubiquitin OS = Avena sativa | 1 | 60 | 57.9 | TLADYNIQK, IQDKEGIPPDQQR, TITLEVESSDTIDNVK | 3 | V |
| 5,(1) | DIM_PEA | Delta(24)-sterol reductase OS = Pisum sativum | 2 | 59 | 45.72 | NILDIDKER, SDLEAPLRPK | 2 | I |
| 8 | HSP11_PEA | 18.1 kDa class I heat shock protein OS = Pisum sativum | 2 | 56 | 8.9 | SIEISG, VLQISGER | 2 | IX |
| 6 | MPPA_SOLTU | Mitochondrial-processing peptidase subunit alpha OS = Solanum tuberosum | 1 | 52 | 3.4 | QLLTYGER, MVASEDIGR | 2 | I |
| 8 | RL17_MAIZE | 60S ribosomal protein L17 OS = Zea mays | 1 | 52 | 10.5 | NAESNADVK, YLEDVIAHK | 2 | V |
| 8 | RL51_ARATH | 60S ribosomal protein L5-1 OS = Arabidopsis thaliana | 2 | 52 | 9.3 | VFGALK, KLTYEER, GALDGGLDIPHSDKR | 3 | V |
| 3 | PHSL1_SOLTU | Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic OS = Solanum tuberosum | 1 | 50 | 1.8 | NDVSYPIK, AFATYVQAK | 2 | II |
| 6 | PRS4A_ARATH | 26S proteasome regulatory subunit 4 homolog A OS = Arabidopsis thaliana | 1 | 49 | 9.5 | VVGSELIQK, GVILYGEPGTGK | 2 | II |
| 8 | YPTC1_CHLRE | GTP-binding protein YPTC1 OS = Chlamydomonas reinhardtii | 2 | 49 | 17.2 | TITSSYYR, LLLIGDSGVGK | 2 | III/VI |
| 4 | HSP7G_ARATH | Heat shock 70 kDa protein 7, chloroplastic OS = Arabidopsis thaliana | 1 | 47 | 9.5 | HIETTLTR, TTPSVVAYTK, QAVVNPENTFFSVKR | 3 | IX |
| 8 | SODM_HEVBR | Superoxide dismutase [Mn], mitochondrial OS = Hevea brasiliensis | 1 | 46 | 11.2 | HHQTYITNYNK, LVVETTANQDPLVTK | 2 | IX |
| 5 | CALX2_ARATH | Calnexin homolog 2 OS = Arabidopsis thaliana | 2 | 45 | 30.3 | NPAYK, SEGHDDYGLLVSEK | 2 | V |
| 8 | RS30_ARATH | 40S ribosomal protein S30 OS = Arabidopsis thaliana | 1 | 44 | 30.6 | GKVHGSLAR, FVTAVVGFGK | 2 | V |
| 8 | RL7A1_ARATH | 60S ribosomal protein L7a-1 OS = Arabidopsis thaliana | 1 | 44 | 9.3 | TLDKNLATSLFK, LKVPPALNQFTK | 2 | V |
| 7 | METK4_POPTR | S-adenosylmethionine synthase 4 OS = Populus trichocarpa | 1 | 42 | 12.3 | FVIGGPHGDAGLTGR, VLVNIEQQSPDIAQGVHGHLTK | 2 | I |
| 8 | RL18A_CASSA | 60S ribosomal protein L18a OS = Castanea sativa | 1 | 41 | 9.6 | ASRPNLFM, FHQYQVVGR | 2 | V |
| 7 | EFTM_ARATH | Elongation factor Tu, mitochondrial OS = Arabidopsis thaliana | 1 | 41 | 9.7 | QAILK, VLAEEGKAK, GITIATAHVEYETAKR | 3 | V |
| 1 | CALSB_ARATH | Callose synthase 11 OS = Arabidopsis thaliana | 1 | 38 | 1.5 | ILFNEAFSR, LGEGKPENQNHALIFTR | 2 | IX |
| 3 | APBLB_ARATH | Beta-adaptin-like protein B OS = Arabidopsis thaliana | 1 | 27 | 2 | EAENIVER, DSQDPNPLIR | 2 | VII |
1 Fraction corresponding to slice of the 1-D gel in which matches for the protein were found. Numbers in parenthesis indicate fractions where additional similar matches (see 2) were found. 2 Number of protein matches of high taxonomical and sequence similarity grouped together with this match. (Match displayed was the top-scored one.) 3 MASCOT score. 4 I: metabolism, II: energy, III: cell growth/division, IV: transcription, V: protein synthesis/destination, VI: transporters, VII: cell structure, VIII: signal transduction, IX: disease/stress defense, and X: unclassified