Table 4.
Spot | Protein | Locus | Function i | Expect. ii | pI | kDa | Fold-Change iii | p Value |
---|---|---|---|---|---|---|---|---|
C1135 | Amino acid ABC transporter substrate-binding protein | Bpr_I1826 | T | 5.8 × 10−4 | 4.0 | 31.6 | Xylan | n/a |
C785 | Aminotransferase DegT/DnrJ/EryC1/StrS family | Bpr_I2311 | I | 1.5 × 10−10 | 5.0 | 51.0 | 4.2 ± 1.1 | 0.001 |
Xylulokinase, XylB | Bpr_I0173 | C | 4.9 | 53.7 | ||||
C1120 | β-Galactosidase, Bga35B | Bpr_I2006 | C | 1.9 × 10−27 | 4.9 | 83.2 | 4.5 ± 0.8 | 0.001 |
β-Xylosidase, Xyl3A | Bpr_I0184 | C | 4.8 | 78.2 | ||||
C704 | Hypothetical protein | Bpr_I2455 | H | 7.6 × 10−22 | 4.7 | 47.7 | 2.3 ± 0.1 | 0.001 |
Phosphoribosylamine-glycine ligase, PurD | Bpr_I0870 | O | 4.7 | 46.3 | ||||
C601 | Oligopeptide ABC transporter substrate-binding protein, OppA1 | Bpr_I1276 | T | 1.9 × 10−13 | 4.0 | 83.1 | 2.3 ± 0.5 | 0.001 |
C784 | Peptidase U62 family | Bpr_I2456 | P | 6.1 × 10−9 | 4.8 | 52.3 | 14.7 ± 4.6 | 0.001 |
C1074 | Sugar ABC transporter substrate-binding protein | Bpr_I0182 | T | 7.6 × 10−7 | 4.12 | 63.3 | Xylan | n/a |
C1057 | Xylose ABC transporter substrate-binding protein | Bpr_I1173 | T | 9.6 × 10−12 | 4.2 | 38.4 | −3.9 ± 1.1 | 0.001 |
i C, Carbohydrate metabolism; H, Hypothetical; I, Cell envelope; O, Nucleotide metabolism; P, Protein fate; T, Transporters; ii Expectation value is the statistical probability of the top ranked protein match being a false positive identification, p < 0.05; iii Fold-change was calculated as the ratio of the mean normalized protein spot volumes in the xylan versus xylose growth conditions as stated. Positive values denote proteins with increased mean normalized spot volume in xylan grown cells. “Xylan” denotes the protein was uniquely detected in culture medium harvested from xylan grown cells. In these cases it was not possible to calculate a p Value and “n/a” (non-applicable) is entered.