Table 1.

N-terminal dimethyl labelled and semi-tryptic peptides identified in Mpn142.

#	Peptide Sequence	Score	E-Value	5600	QE
	N-terminal Dimethyl Labelled
N1	-.1MKSKLKLKR9.Y	11 *	0.72	1	1
N2	L.25ANTYLLQDHNTLTPYTPFTTPLNGGLDVVR54.A	36	0.0024	1	3
N3	A.26NTYLLQDHNTLTPYTPFTTPLNGGLDVVR54.A	142	3.5 × 10−13	4	4
N4	R.153KIKLALPYVKQESQGSGDQGSNGKGSLYKTLQDLLVEQPVTPYTPNAGLARVNGV207.A	66	2.3 × 10−5	-	1
N5	R.368TASDTATFSK377.Y	50	0.00068	-	1
N6	R.368TASDTATFSKYLNTAQALHQMGVIVPGLEKWGGNNGTGVVAS409.R	55	1.2 × 10−5	2	-
N7	R.368TASDTATFSKYLNTAQALHQMGVIVPGLEKWGGNNGTGVVASR410.Q	146	1.1 × 10−13	2	3
N8	R.368TASDTATFSKYLNTAQALHQMGVIVPGLEKWGGNNGTGVVASRQ411.D	148	5.2 × 10−14	1	4
N9	R.368TASDTATFSKYLNTAQALHQMGVIVPGLEKWGGNNGTGVVASRQD412.A	163	1.2 × 10−15	-	2
N10	R.368TASDTATFSKYLNTAQALHQMGVIVPGLEKWGGNNGTGVVASRQDA413.T	80	9.8 × 10−8	-	2
N11	R.368TASDTATFSKYLNTAQALHQMGVIVPGLEKWGGNNGTGVVASRQDAT414.S	62	3.9 × 10−6	-	2
N12	T.369ASDTATFSKYLNTAQALHQMGVIVPGLEKWGGNNGTGVVASR410.Q	138	2.7 × 10−13	2	4
N13	R.446AVTVVAGPLR455.A	72	1.2 × 10−5	3	4
N14	A.447VTVVAGPLR455.A	48	0.0024	3	4
N15	L.696GLNFNFKLNEER707.L	13 *	1.3	2	2
N16	R.731ENAQSTSDDNSNTKVKWTNTASHYLPVPYYYSANFPEAGNRR772.R	21 *	0.015	-	1
N17	A.775EQRNGVKISTLESQATDGFANSLLNFGTGLKAGVDPAPVAR815.G	41	0.00025	-	1
N18	A.1022NSVLQARNLTDKTVDEVINNPDILQSFFKFTPAFDNQR1059.A	84	8.8 × 10−7	3	1
N19	K.1190AANNAAPKAPVKPAAPTAPRPPVQPPKKA1218.-	59	4.7 × 10−6	-	1
N20	A.1191ANNAAPKAPVKPAAPTAPRPPVQPPKKA1218.-	99	1.7 × 10−9	1	3
N21	A.1192NNAAPKAPVKPAAPTAPRPPVQPPKKA1218.-	41	0.00014	-	1
N22	N.1194AAPKAPVKPAAPTAPRPPVQPPKKA1218.-	44	0.00019	-	2
N23	A.1195APKAPVKPAAPTAPRPPVQPPKKA1218.-	18 *	0.051	-	1
N24	P.1197KAPVKPAAPTAPRPPVQPPKKA1218.-	21 *	0.029	-	1
	Semi-tryptic Truncated C-terminal Peptides
S1	R.253KLNESWPVYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAA301.G	73	5.4 × 10−7	1	-
S2	R.253KLNESWPVYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAAGS303.A	87	1.4 × 10−8	1	-
S3	R.253KLNESWPVYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAAGSA304.S	30 *	0.0071	1	-
S4	R.253KLNESWPVYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAAGSAS305.S	40	0.00072	1	-
S5	R.253KLNESWPVYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAAGSASS306.L	39	0.00077	-	1
S6	R.253KLNESWPVYEPLDSTKEGKGKDESSWKNSEKTTAENDAPLVGMVGSGAAGSASSLQ308.G	86	1.4 × 10−7	1	-
S7	R.1029NLTDKTVDEVINNPDILQSFFKFTPAFDNQRAMLV1063.G	45	0.0023	-	1
	Semi-tryptic Truncated N-terminal Peptides
S8	D.810PAPVARGHKPNYSAVLLVR828.G	65	2.3 × 10−6	-	1
S9	N.983GLFEKDDQLSENVKRR998.D	26 *	0.033	-	1
S10	N.1193NAAPKAPVKPAAPTAPRPPVQPPKKA1218.-	25 *	0.015	-	1
	P1 adhesin Cleaved Peptides
C1	R.1594LKQTSAAKPG1603.A (Semi-tryptic)	24 *	0.045	-	1
C2	T.1598SAAKPGAPRPPVPPKPGAPKPPVQPPKKPA1627.- (Dimethyl labeled)	58	4.5 × 10−6	-	2

All peptides have a score >32 and an E-value < 0.05 unless indicated by * which signifies that the peptide was either identified over several replicates or correlates with predicted fragments in this study. # indicates number. Cleavage sites are located beside the bold underlined amino acid (left for N-terminus and right for C-terminus). Amino acid number is written as superscript at the start and end of the peptide. Highest ion score and lowest E-value for the peptide identified across the replicates is listed. The last two columns contain the number of times the peptide was identified by either the Sciex 5600 TripleTOF or Thermo Q Exactive Plus out of a total of six biological replicates. The last two peptides are C-terminal cleavage sites identified for P1 (Uniprot #: P11311).