Fig. 5.

qRT-PCR-based validation of the transcription expression of twelve genes selected from the DGE analysis. a Ca²⁺/H⁺-exchanging protein. b AAA-type ATPase. c NRT1/PTR-like transporter. d Auxin response factor 6-like. e Auxin induced protein PCNT115. f Ascorbate peroxidase. g Ethylene responsive factor 5a. h DNA damage-binding protein 1a. i DNA repair protein RAD23. j PP2A regulatory subunit TAP46. k MADS-box transcription factor TaAGL4. l MADS-box transcription factor TaAGL33. The qRT-PCR data are given in the form mean ± s.d. (n = 3). The asterisk and double asterisks represent significant difference determined by the Student’s t-test at P < 0.05 (*) and P < 0.01 (**), respectively