Fig. 5.

Casticin inhibits tumor growth in vivo. a Different concentrations (10% DMSO + 90% PBS, 10 and 20 mg/kg) casticin were injected into nude mice after inoculated NOZ cells every 2 days. Images of 5 representative mice (n = 7) from each group are presented to show the sizes of the resulting tumors. b Tumors were excised from the animals and weighed. c–e Bcl-2, cyclinD1, p-AKT and Ki-67 expression levels were analyzed using HE, IHC staining and western blot analysis. f Representative images of TUNEL assay of tumor xenografts (original magnifications: ×400). All data are presented as the means ± standard deviations. Significant differences from the control were indicated by *p < 0.05, **p < 0.01, and ***p < 0.001