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. Author manuscript; available in PMC: 2017 Mar 11.
Published in final edited form as: Science. 2016 Mar 10;351(6278):1204–1208. doi: 10.1126/science.aac5610

Figure 2. MDMX suppresses p53 oscillations in non-stressed conditions and after DNA damage.

Figure 2

(A) Schematic of the p53 and MDMX reporter constructs.

(B) Abundance of mKate2-MDMX in p53-MDMX reporter cells treated with doxycycline analyzed by western blot.

(C and D) Time-lapse microscopy images of p53-mCerulean (C) and mKate2-MDMX (D) after MDMX siRNAs (30hr), followed by doxycycline.

(E and F) Trajectories of p53-mCerulean and mKate2-MDMX levels are shown for cells with either MDMX knockdown (E) or NCS (F) treatment 30 hr prior to doxycycline addition.

(G and H) Trajectories of p53-mCerulean levels are shown for cells treated with NCS alone (G) or with prior treatment of doxycycline (H).