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. 2016 Sep 14;3(1):82–98. doi: 10.1016/j.jcmgh.2016.09.001

Figure 7.

Figure 7

Generation and validation of a mouse with endothelial cell specific CD36 deletion. (A) A mouse with endothelial cell deletion of CD36 (EC-CD36KO) was generated by breeding floxed (Fl/Fl) CD36 mice with mice carrying the Tie2-Cre recombinase (Cre+ males with Cre– females). PCR of DNA isolated from liver, muscle, and lung tissues of EC-CD36KO and floxed mice showing presence of null allele in EC-CD36KO mice. (B) Staining of intestinal villi from EC-CD36KO and Fl/Fl control mice with CD31 (marker of endothelial cells) and CD36. Insert: Blowup of areas indicated by white arrows showing CD36 expression in CD31+ cells in Fl/Fl but not in EC-CD36KO mice; scale bar: 30 μm. (C) CD36 protein levels in lysates from proximal intestines showing average 38% decrease in EC-CD36KO as compared with Fl/Fl mice (germline CD36KO mice are negative controls) (P = .05). (D) CD31 and CD36 mRNA expression in endothelial cells (CD146+) isolated from lungs of Fl/Fl and EC-CD36KO mice (n = 3/genotype). CD36 mRNA levels are reduced in CD146+ cells from EC-CD36KO mice as compared with Fl/Fl controls (P < .01), whereas CD31 mRNA levels are similar. Graphs show data as means ± SEM; n = 3/genotype.