Fig. 3.

a TB activities of anti-rPSOP25 serum and anti-rPSOP25 mAb on P. berghei ookinete formation in vitro. Anti-rPSOP25 serum, anti-rPSOP25 mAb, or normal mouse serum (control) were diluted at 1:5, 1:10 and 1:50, respectively. Means were representative of three independent experiments. Error bar shows mean ± SD. ** indicate significant difference compared with the control sera (P < 0.01). # indicate significant difference between anti-rPSOP25 serum and mAb group (P < 0.05), ## P < 0.01 (Student’s t-test). b Direct mosquito feeding assay to assess the TB activity of polyclonal antisera in rPSOP25-immunized mice (3 mice per group). c Passive antibody transfer experiment to assess the TB activity of the anti-PSOP25 mAb. For b and c, mosquito midguts were dissected at ten days post-infection, the number of oocysts was counted under a microscopy. The data are collated from three experiments. The mean number of oocysts and the SEM in each group are shown. **P < 0.01 (Mann-Whitney U-test)