Table 2.
Kinetic constants for phosphorylation of p27KIP1 peptides
| Peptide | Sequence | Km [μM] | Relative rate [pmol/min] |
Relative rate/Km |
|---|---|---|---|---|
| wt | N180AGSVEQTPKKPGLR | 93.6 | 2.21 | 0.0195 |
| R194A | N180AGSVEQTPKKPGLA | 81.4 | 1.11 | 0.0135 |
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