FIGURE 6.
CUA-1.1 sequesters excess copper to gut granules. A, confocal images of transgenic animals (Pvha-6::CUA-1.1::GFP::unc-54 3′UTR) exposed to varying RNAi conditions in the presence of 50 μm supplemental copper, followed by incubation with LysoTracker. Boxed images in the top row are enlarged at the corner of each panel. Scale bar, 10 μm. B, synchronized wild-type L1 larvae were cultured on RNAi plates supplemented with the indicated concentrations of copper for 2.5 days. TOF was determined using a COPAS Biosort. Error bars indicate average ± S.E. of two independent experiments. (**, p < 0.01, two-way ANOVA, Tukey's post hoc test.) C, total copper levels of wild-type and pgp-2(kx48) worms as measured by ICP-MS. Error bars show mean ± S.E. of three independent experiments. Values marked with different letters are significantly different at p = 0.05 (two-way ANOVA, Tukey's post hoc test).